Expression and Characterization of Three New Glutathione Transferases, an Epsilon (AcGSTE2-2), Omega (AcGSTO1-1), and Theta (AcGSTT1-1) from Anopheles cracens (Diptera: Culicidae), a Major Thai Malaria Vector

@inproceedings{Wongtrakul2010ExpressionAC,
  title={Expression and Characterization of Three New Glutathione Transferases, an Epsilon (AcGSTE2-2), Omega (AcGSTO1-1), and Theta (AcGSTT1-1) from Anopheles cracens (Diptera: Culicidae), a Major Thai Malaria Vector},
  author={J. Wongtrakul and S. Pongjaroenkit and P. Leelapat and Woottichai Nachaiwieng and L. Prapanthadara and A. Ketterman},
  booktitle={Journal of medical entomology},
  year={2010}
}
ABSTRACT Glutathione transferases (GSTs) (E.C.2.5.1.18) are multifunctional enzymes involved in the detoxification of many exogenous and endogenous compounds. This study aimed to characterize several new GSTs from Anopheles cracens, a major Thai malaria vector formerly known as Anopheles dirus. The three recombinant enzymes obtained were from the epsilon, theta and omega classes. They showed 80–93% identity to orthologous An. gambiae GSTs. AcGSTE2-2 possessed peroxidase activity that cannot be… Expand
Comparative biochemical characterization of Drosophila Melanogaster epsilon class glutathione s-transferase, DmGSTE6 and DmGSTE7 / Vennobaahshini a/p Venu
TLDR
The study compares the biochemical behavior of two epsilon class Glutathione S- Transferase (GSTs) genes from Drosophila melanogaster, namely gste6 and gste7, and reveals that phenol red dye possessed inhibition effects only on GSTE6 while rose bengal and cardiogreen dye inhibit excellently both G STE6 and GSTE7. Expand
Identification and expression profiles of nine glutathione S-transferase genes from the important rice phloem sap-sucker and virus vector Laodelphax striatellus (Fallén) (Hemiptera: Delphacidae).
TLDR
This work is the first identification of GST genes from different GST groups in Auchenorrhyncha species and their induction characteristics with insecticide types and time and might be related to the enhanced tolerance of this insect to insecticides and xenobiotics. Expand
Identification of Putative Carboxylesterase and Glutathione S-transferase Genes from the Antennae of the Chilo suppressalis (Lepidoptera: Pyralidae)
TLDR
This study identified 19 putative CXE and 16 GST genes by analyzing previously constructed antennal transcriptomes of C. suppressalis, an economically important lepidopteran pest which causes great economic damage to cultivated rice crops in many Asian countries, and found none of the GST genes were found to be antennae-specific. Expand
GLUTATHIONE S-TRANSFERASE Genes IN THE RICE LEAFFOLDER, Cnaphalocrocis medinalis (LEPIDOPTERA: PYRALIDAE): IDENTIFICATION AND EXPRESSION PROFILES.
TLDR
Real-time quantitative PCR analysis showed that these GST genes were differentially expressed in various tissues, including the midgut, Malpighian tubules, and fat body of larvae, and the antenna, abdomen, and leg of adults, indicating diversified functions for these genes. Expand
Characterization and expression profiling of glutathione S-transferases in the diamondback moth, Plutella xylostella (L.)
TLDR
This is the most comprehensive study on genome-wide identification, characterization and expression profiling of the GST family in P. xylostella and provides a base for functional research on specific GST genes, a better understanding of the evolution of insecticide resistance, and strategies for more sustainable management of the pest. Expand
Genomic Insights into the Glutathione S-Transferase Gene Family of Two Rice Planthoppers, Nilaparvata lugens (Stål) and Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)
TLDR
This study provides the first elucidation of the nature of the GST gene family in a delphacid species, offering new insights into the evolution of metabolic enzyme genes in insects. Expand
Interaction of Omega, Sigma, and Theta glutathione transferases with p38b mitogenactivated protein kinase from the fruit fly, Drosophila melanogaster
TLDR
A novel link between Omega and Theta GSTs with the p38b MAP kinase pathway is suggested, including the reciprocal effect of this kinase protein on glutathione transferase activity. Expand
Interaction of Omega, Sigma, and Theta Glutathione Transferases with p38b Mitogen-Activated Protein Kinase from the Fruit Fly, Drosophila melanogaster
Abstract Glutathione S-transferases (GSTs) are a diverse family of phase II detoxification enzymes found in almost all organisms. Besides playing a major role in the detoxification of xenobiotic andExpand
Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress
TLDR
CsGSTos might be critically involved in protection of the reproductive system during maturation of C. sinensis worms and in response to oxidative conditions, thereby contributing to maintenance of parasite fecundity. Expand
Phylogenetic analysis of the GST family in Anopheles (Nyssorhynchus) darlingi.
Anopheles darlingi Root, 1926 and Anopheles gambiae (Diptera: Culicidae) are the most important human malaria vectors in South America and Africa, respectively. The two species are estimated to haveExpand
...
1
2
...

References

SHOWING 1-10 OF 30 REFERENCES
Characterization of Anopheles dirus Glutathione Transferase Epsilon 4
TLDR
It is concluded that adG STE4-4, unlike agGSTE4- 4, can contribute to DDT resistance by DDT dehydrochlorinase activity as well as to pyrethroid resistance by sequestration and protection against oxidation from secondary pyrethoid metabolites via its peroxidase activity. Expand
Identification, characterization and structure of a new Delta class glutathione transferase isoenzyme.
TLDR
A crystal structure was obtained for adGSTD5-5 and compared it with other Delta GSTs, which showed that it possesses an elongated and more polar active-site topology, which suggests that the enzyme may be modulated by fatty acids. Expand
Expression and characterization of a novel class of glutathione S-transferase from Anopheles dirus.
TLDR
Enzyme characterization of adGST4-1 shows it to be distinct from the other An. Expand
Cloning, expression and characterization of theta-class glutathione S-transferase from the silkworm, Bombyx mori.
TLDR
This study focused on glutathione S-transferase (GST), one of the detoxification enzymes, from the silkworm, Bombyx mori, which was functionally overexpressed in Escherichia coli in a soluble form, purified to homogeneity, and characterized. Expand
Heterologous expression and characterization of alternatively spliced glutathione S-transferases from a single Anopheles gene.
TLDR
Despite the enzymes being splicing products of the same gene and sharing identical sequence in the N-terminal 45 amino acids, these GSTs show distinct substrate specificities, kinetic properties and inhibition properties modulated by the differences in the C-terminus. Expand
Heterologous expression of four glutathione transferase genes genetically linked to a major insecticide-resistance locus from the malaria vector Anopheles gambiae.
TLDR
A cluster of eight genes encoding glutathione transferases located on division 33B of polytene chromosome arm 3R of the African malaria mosquito, Anopheles gambiae, indicated that the expression of GSTE2-2 is elevated in a DDT-resistant strain of A. gambiae. Expand
Identification and cloning of a key insecticide-metabolizing glutathione S-transferase (MdGST-6A) from a hyper insecticide-resistant strain of the housefly Musca domestica.
TLDR
The purification and identification of a M. domestica GST isozyme broadly specific for substrates from a housefly strain, Cornell-HR, that is highly resistant against OP-insecticides, and the isolation of two new MdGST genes using the antibody made against it are described. Expand
Elevated activity of an Epsilon class glutathione transferase confers DDT resistance in the dengue vector, Aedes aegypti.
TLDR
It is shown that the putative ortholog of GSTe2 in Ae. Expand
Catalytic and structural contributions for glutathione-binding residues in a Delta class glutathione S-transferase.
TLDR
It was concluded that these residues contribute to the affinity of the GSH-binding site ('G-site') for GSH, influence GSH thiol ionization, participate in kcat regulation by affecting the rate-limiting step of the reaction, and in the case of Ile-52 and Arg-66, influenced structural integrity and/or folding of the enzyme. Expand
Role of Yeast Glutaredoxins as Glutathione S-transferases*
TLDR
Glutaredoxins are multifunctional enzymes with oxidoreductase, peroxidase, and GST activity, and are therefore ideally suited to detoxify the wide range of xenobiotics and oxidants that can be generated during diverse stress conditions. Expand
...
1
2
3
...