Expression and Characterization of Three New Glutathione Transferases, an Epsilon (AcGSTE2-2), Omega (AcGSTO1-1), and Theta (AcGSTT1-1) from Anopheles cracens (Diptera: Culicidae), a Major Thai Malaria Vector

@inproceedings{Wongtrakul2010ExpressionAC,
  title={Expression and Characterization of Three New Glutathione Transferases, an Epsilon (AcGSTE2-2), Omega (AcGSTO1-1), and Theta (AcGSTT1-1) from Anopheles cracens (Diptera: Culicidae), a Major Thai Malaria Vector},
  author={Jeerang Wongtrakul and Saengtong Pongjaroenkit and Posri Leelapat and Woottichai Nachaiwieng and La-aied Prapanthadara and Albert J. Ketterman},
  booktitle={Journal of medical entomology},
  year={2010}
}
ABSTRACT Glutathione transferases (GSTs) (E.C.2.5.1.18) are multifunctional enzymes involved in the detoxification of many exogenous and endogenous compounds. This study aimed to characterize several new GSTs from Anopheles cracens, a major Thai malaria vector formerly known as Anopheles dirus. The three recombinant enzymes obtained were from the epsilon, theta and omega classes. They showed 80–93% identity to orthologous An. gambiae GSTs. AcGSTE2-2 possessed peroxidase activity that cannot be… 

Comparative biochemical characterization of Drosophila Melanogaster epsilon class glutathione s-transferase, DmGSTE6 and DmGSTE7 / Vennobaahshini a/p Venu

The study compares the biochemical behavior of two epsilon class Glutathione S- Transferase (GSTs) genes from Drosophila melanogaster, namely gste6 and gste7, and reveals that phenol red dye possessed inhibition effects only on GSTE6 while rose bengal and cardiogreen dye inhibit excellently both G STE6 and GSTE7.

Identification and expression profiles of nine glutathione S-transferase genes from the important rice phloem sap-sucker and virus vector Laodelphax striatellus (Fallén) (Hemiptera: Delphacidae).

This work is the first identification of GST genes from different GST groups in Auchenorrhyncha species and their induction characteristics with insecticide types and time and might be related to the enhanced tolerance of this insect to insecticides and xenobiotics.

Identification of Putative Carboxylesterase and Glutathione S-transferase Genes from the Antennae of the Chilo suppressalis (Lepidoptera: Pyralidae)

This study identified 19 putative CXE and 16 GST genes by analyzing previously constructed antennal transcriptomes of C. suppressalis, an economically important lepidopteran pest which causes great economic damage to cultivated rice crops in many Asian countries, and found none of the GST genes were found to be antennae-specific.

GLUTATHIONE S-TRANSFERASE Genes IN THE RICE LEAFFOLDER, Cnaphalocrocis medinalis (LEPIDOPTERA: PYRALIDAE): IDENTIFICATION AND EXPRESSION PROFILES.

Real-time quantitative PCR analysis showed that these GST genes were differentially expressed in various tissues, including the midgut, Malpighian tubules, and fat body of larvae, and the antenna, abdomen, and leg of adults, indicating diversified functions for these genes.

Characterization and expression profiling of glutathione S-transferases in the diamondback moth, Plutella xylostella (L.)

This is the most comprehensive study on genome-wide identification, characterization and expression profiling of the GST family in P. xylostella and provides a base for functional research on specific GST genes, a better understanding of the evolution of insecticide resistance, and strategies for more sustainable management of the pest.

Genomic Insights into the Glutathione S-Transferase Gene Family of Two Rice Planthoppers, Nilaparvata lugens (Stål) and Sogatella furcifera (Horváth) (Hemiptera: Delphacidae)

This study provides the first elucidation of the nature of the GST gene family in a delphacid species, offering new insights into the evolution of metabolic enzyme genes in insects.

Interaction of Omega, Sigma, and Theta glutathione transferases with p38b mitogenactivated protein kinase from the fruit fly, Drosophila melanogaster

A novel link between Omega and Theta GSTs with the p38b MAP kinase pathway is suggested, including the reciprocal effect of this kinase protein on glutathione transferase activity.

Interaction of Omega, Sigma, and Theta Glutathione Transferases with p38b Mitogen-Activated Protein Kinase from the Fruit Fly, Drosophila melanogaster

A novel link between Omega and Theta GSTs with the p38b MAP kinase pathway is suggested, including the reciprocal effect of this kinase protein on glutathione transferase activity.

Clonorchis sinensis omega-class glutathione transferases play major roles in the protection of the reproductive system during maturation and the response to oxidative stress

CsGSTos might be critically involved in protection of the reproductive system during maturation of C. sinensis worms and in response to oxidative conditions, thereby contributing to maintenance of parasite fecundity.

References

SHOWING 1-10 OF 30 REFERENCES

Characterization of Anopheles dirus Glutathione Transferase Epsilon 4

It is concluded that adG STE4-4, unlike agGSTE4- 4, can contribute to DDT resistance by DDT dehydrochlorinase activity as well as to pyrethroid resistance by sequestration and protection against oxidation from secondary pyrethoid metabolites via its peroxidase activity.

Identification, characterization and structure of a new Delta class glutathione transferase isoenzyme.

A crystal structure was obtained for adGSTD5-5 and compared it with other Delta GSTs, which showed that it possesses an elongated and more polar active-site topology, which suggests that the enzyme may be modulated by fatty acids.

Cloning, expression and characterization of theta-class glutathione S-transferase from the silkworm, Bombyx mori.

Heterologous expression of four glutathione transferase genes genetically linked to a major insecticide-resistance locus from the malaria vector Anopheles gambiae.

A cluster of eight genes encoding glutathione transferases located on division 33B of polytene chromosome arm 3R of the African malaria mosquito, Anopheles gambiae, indicated that the expression of GSTE2-2 is elevated in a DDT-resistant strain of A. gambiae.

Catalytic and structural contributions for glutathione-binding residues in a Delta class glutathione S-transferase.

It was concluded that these residues contribute to the affinity of the GSH-binding site ('G-site') for GSH, influence GSH thiol ionization, participate in kcat regulation by affecting the rate-limiting step of the reaction, and in the case of Ile-52 and Arg-66, influenced structural integrity and/or folding of the enzyme.

Role of Yeast Glutaredoxins as Glutathione S-transferases*

Glutaredoxins are multifunctional enzymes with oxidoreductase, peroxidase, and GST activity, and are therefore ideally suited to detoxify the wide range of xenobiotics and oxidants that can be generated during diverse stress conditions.