Expression analysis of surface molecules on human thymic dendritic cells with the 10th HLDA Workshop antibody panel

  title={Expression analysis of surface molecules on human thymic dendritic cells with the 10th HLDA Workshop antibody panel},
  author={Stephanie Gurka and Stefan Dirks and Joachim Photiadis and Richard A. Kroczek},
  journal={Clinical \& Translational Immunology},
Dendritic cells (DC) in the thymus have an important role in the establishment of central tolerance by promoting negative selection of autoreactive T cells and regulatory T‐cell differentiation. Whereas human DC have recently been studied in various tissues in more detail, thymic DC subsets are still ill‐defined. In the present work, we studied the binding of 71 monoclonal antibodies (mAb) submitted to the HLDA10 workshop to human CD123+ plasmacytoid DC and the two subsets of conventional DC… Expand
9 Citations
Transcriptomes of antigen presenting cells in human thymus
It is shown that thymic CD141+ DCs express the highest levels of all classical HLA genes and 67% (14/21) of the HLA class I and II pathway genes investigated in this study. Expand
Dendritic cell subsets.
This review outlines mouse and human steady state DC subsets and key attributes that define their distinct functions and describes the role of different DC in health and disease. Expand
Generation of mature T cells from human hematopoietic stem/progenitor cells in artificial thymic organoids
A serum-free, artificial thymic organoid (ATO) system that supports efficient and reproducible in vitro differentiation and positive selection of conventional human T cells from all sources of HSPCs is described. Expand
Dendritic cell subsets and locations.
Dendritic cells are a unique class of immune cells that act as a bridge between innate and adaptive immunity, and are classified into different subsets based on an integrated approach considering their surface phenotype, expression of unique and conserved molecules, ontogeny, and functions. Expand
Tim-3 is a Marker of Plasmacytoid Dendritic Cell Dysfunction during HIV Infection and Is Associated with the Recruitment of IRF7 and p85 into Lysosomes and with the Submembrane Displacement of TLR9
It is concluded that Tim-3 is a biomarker of dysfunctional pDCs and may negatively regulate IFN-α, possibly through interference with TLR signaling and recruitment of IRF7 and p85 into lysosomes, enhancing their degradation. Expand
Thymus and autoimmunity
The thymus prevents autoimmune diseases through mechanisms that operate in the cortex and medulla, comprising positive and negative selection and the generation of regulatory T-cells (Tregs) and polymorphisms and mutations of tolerance-related genes with an impact on positive selection are covered. Expand
Sensing Tissue Damage by Myeloid C-Type Lectin Receptors.
This chapter explores how myeloid cells develop their sentinel function, exploring how some of these CLRs identify cell death and the type of responses triggered thereof, and focuses on DNGR-1, Mincle, and CLL-1 as paradigmatic death-sensing CLRs expressed by myeloids cells. Expand
Thyrotropin Alters T Cell Development in the Thymus in Subclinical Hypothyroidism Mouse Model
Results suggest that TSH promoted T cell development and enhanced the thymic recent output in SCH mice, possibly by suppression of apoptosis of thymocytes, indicating that modification of the ERK signalling pathways. Expand
Constitutive and activation-dependent phosphorylation of lymphocyte phosphatase-associated phosphoprotein (LPAP)
The results confirmed previous reports that Ser-99, Ser-153, and Ser-163 are phosphorylated, as well as provided evidence for the phosphorylation of Ser-172, and suggest that LPAP may function as a co-regulator of T-cell signaling. Expand


Analysis of HLDA9 mAbs on plasmacytoid dendritic cells.
The surface reactivity of a panel of 96 monoclonal antibodies submitted to the ninth Human Leukocyte Differentiation Antigens workshop (HLDA9) B cell section was analyzed and revealed the expression of eight surface molecules not previously described on pDCs, including CD352(NTBA), CD272(BTLA), CD357(GITR), CD48, CD270, Galectin-3, CD148, and CD361. Expand
Screening of the HLDA9 panel on peripheral blood dendritic cell populations.
Drawing parallels between the CD11c(+) DC subsets and the relatively immature state of peripheral blood DC was highlighted and some of the mAb expected to bind to DC populations failed in this analysis. Expand
Ex vivo characterization of human thymic dendritic cell subsets.
An in vitro evolution of human thymic pDC toward a myeloid phenotype found in the CD11c(+) subset of thymo-dendritic DC is suggested. Expand
Human CD141+ (BDCA-3)+ dendritic cells (DCs) represent a unique myeloid DC subset that cross-presents necrotic cell antigens
The data demonstrate a role for CD141+ DCs in the induction of cytotoxic T lymphocyte responses and suggest that they may be the most relevant targets for vaccination against cancers, viruses, and other pathogens. Expand
Superior antigen cross-presentation and XCR1 expression define human CD11c+CD141+ cells as homologues of mouse CD8+ dendritic cells
It is shown that CD141+ DCs are the only cells in human blood that express the chemokine receptor XCR1 and respond to the specific ligand XCL1 by Ca2+ mobilization and potent chemotaxis and defined as professional antigen cross-presenting DCs in the human. Expand
Expression of XCR1 Characterizes the Batf3-Dependent Lineage of Dendritic Cells Capable of Antigen Cross-Presentation
XCR1 emerges as the first surface marker characterizing a DC lineage in the mouse and potentially also in the human, and it is demonstrated that XCR1+ DCs in the spleen, LNs, and peripheral tissues are dependent on the growth factor Flt3 ligand and are selectively absent in Batf3-deficient animals. Expand
Human thymus contains 2 distinct dendritic cell populations.
WhereasCD11b+ thymic DCs appear to be related to tonsillar germinal-center DCs, the major CD11b- IL-12-secreting human thymus DC population has similarities to mouse CD 11b- CD8+ DCs. Expand
Human Tissues Contain CD141hi Cross-Presenting Dendritic Cells with Functional Homology to Mouse CD103+ Nonlymphoid Dendritic Cells
A CD141hi DC present in human interstitial dermis, liver, and lung that was distinct from the majority of CD1c+ and CD14+ tissue DCs and superior at cross-presenting soluble antigens was identified. Expand
Human thymus contains IFN-alpha-producing CD11c(-), myeloid CD11c(+), and mature interdigitating dendritic cells.
Three distinct dendritic cell (DC) subsets capable of stimulating allogeneic naive T cells were isolated from human thymus and the detection of Spi-B mRNA, not only upon in vitro maturation of pDCs, but also in freshly purified IDCs, suggests that in vivo p DCs may differentiate into IDCs. Expand
A discrete population of IFN λ‐expressing BDCA3hi dendritic cells is present in human thymus
In human thymus the presence of a discrete population of BDCA3hi DCs that, like their peripheral counterparts, express CD13, low‐intermediate levels of CD11c, CLEC9A, high levels of XCR1, IRF8 and TLR3 are described, which indicates that, similarly to thymic pDCs, BD CA3hiDCs can have an important role in thymUS protection against viral infections. Expand