Expression Vectorion Construction of Hepatitis B Surface antigen(HBsAg) Gene and Transformation to Crowtoe(Lotus corniculatus)

@inproceedings{Yingshan2007ExpressionVC,
  title={Expression Vectorion Construction of Hepatitis B Surface antigen(HBsAg) Gene and Transformation to Crowtoe(Lotus corniculatus)},
  author={Dong Ying-shan},
  year={2007}
}
To construct the high effect expression plasmid, the Hepatitis B surface antigen (HBsAg) gene was cloned from Hepatitis B virus DNA by using PCR method, and it was inserted into vector of pBin438 .The plant expression vector pBHB was obtained. Crowtoes(Lotus corniculatus)transformed by Agrobacterium-mediated method were analyzed for the integration of the transgene by PCR and Southern blot. The positive band indicated that the exterior gene had been integrated into crowtoe. There were 20… CONTINUE READING

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