Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients

@article{Kobayashi2008ExpressionPO,
  title={Expression Profiling of PBMC-based Diagnostic Gene Markers Isolated from Vasculitis Patients},
  author={Shigeto Kobayashi and Akihiko Ito and Daisuke Okuzaki and Hiroaki Onda and N. Harumi Yabuta and Ippei Nagamori and Kazuo Suzuki and Hiroshi Hashimoto and Hiroshi Nojima},
  journal={DNA Research: An International Journal for Rapid Publication of Reports on Genes and Genomes},
  year={2008},
  volume={15},
  pages={253 - 265}
}
Vasculitis (angiitis) is a systemic autoimmune disease that often causes fatal symptoms. We aimed to isolate cDNA markers that would be useful for diagnosing not only vasculitis but also other autoimmune diseases. For this purpose, we used stepwise subtractive hybridization and cDNA microarray analyses to comprehensively isolate the genes whose expressions are augmented in peripheral blood mononuclear cells (PBMCs) pooled from vasculitis patients. Subsequently, we used quantitative real-time… CONTINUE READING
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