Expression, purification and use of recombinant annexin V for the detection of apoptotic cells

@article{Logue2009ExpressionPA,
  title={Expression, purification and use of recombinant annexin V for the detection of apoptotic cells},
  author={Susan E Logue and Mohamed Elgendy and Seamus J. Martin},
  journal={Nature Protocols},
  year={2009},
  volume={4},
  pages={1383-1395}
}
Apoptosis is a mode of programmed cell death that is widely used to eliminate cells during development, tissue homeostasis, infection or in response to injury. Alterations to the plasma membranes of apoptotic cells trigger recognition and engulfment of such cells by phagocytes. Measurement of plasma membrane phosphatidylserine externalization, using fluorescently labeled annexin V, is widely used for the detection of apoptotic cells. Here we describe protocols for bacterial expression… CONTINUE READING

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