Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a beta-agarase from the flavobacterium Zobellia galactanivorans.

@article{Hehemann2010ExpressionPA,
  title={Expression, purification and preliminary X-ray diffraction analysis of the catalytic module of a beta-agarase from the flavobacterium Zobellia galactanivorans.},
  author={Jan Hendrik Hehemann and Gurvan Michel and Tristan Barbeyron and Mirjam Czjzek},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2010},
  volume={66 Pt 4},
  pages={413-7}
}
Marine bacteria secrete specific glycoside hydrolases such as agarases to access polysaccharides from algal cell walls as a carbon and energy source. In an attempt to identify agarases with variable degradation patterns, a novel family GH16 beta-agarase from the marine bacterium Zobellia galactanivorans was expressed, purified and crystallized. The purified enzyme crystallized in two distinct forms that were grown by the hanging-drop vapour-diffusion method using polyethylene glycol as a… CONTINUE READING

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