Expression, purification and ligand binding properties of the recombinant translation initiation factor (PeIF5B) from Pisum sativum

@article{Rasheedi2010ExpressionPA,
  title={Expression, purification and ligand binding properties of the recombinant translation initiation factor (PeIF5B) from Pisum sativum},
  author={Sheeba Rasheedi and Madhuri Suragani and Soghra Khatun Haq and Sachchidanand and Rajesh Bhardwaj and Seyed E. Hasnain and Nasreen Zafar Ehtesham},
  journal={Molecular and Cellular Biochemistry},
  year={2010},
  volume={344},
  pages={33-41}
}
Gene encoding a novel translation initiation factor PeIF5B from Pisum sativum with sequence similarity to eIF5B from H. sapiens, D. melanogaster, S. cerevisiae as well as archaeal aIF5B from M. thermoautotrophicum was earlier reported by us. We now describe the expression and purification of 96 kDa recombinant PeIF5B (rPeIF5B) protein. Using fluorescence and circular dichroism spectra analyses, we show that Mg2+ binding does not lead to any change in PeIF5B aromatic amino acid micro-environment… CONTINUE READING
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PROCHECK: a program to check the stereochemical quality of protein structures

  • RA Laskowski, MW MacArthur, DS Moss, JM Thornton
  • J Appl Cryst
  • 1993
Highly Influential
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