Expression, purification and characterization of recombinant human proinsulin.

  title={Expression, purification and characterization of recombinant human proinsulin.},
  author={David J Cowley and Robert B. Mackin},
  journal={FEBS letters},
  volume={402 2-3},
We have recently developed a method to produce native human proinsulin using a bacterial expression system. A proinsulin fusion protein was recovered from inclusion bodies and cleaved using cyanogen bromide. The released proinsulin polypeptide was S-sulfonated and purified by anion exchange chromatography. Following refolding, proinsulin was purified by reversed-phase high-performance liquid chromatography. Combined peptide mapping and mass spectrometric analysis indicated that the proinsulin… CONTINUE READING
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