Expression, purification, refolding, and enzymatic characterization of two secretory phospholipases A₂ from Neurospora crassa.

Abstract

Secretory phospholipase A2 (sPLA2) catalyzes the hydrolysis of sn-2 linkage in the glycerophospholipid, thereby releasing fatty acid and 1-acyl lysophospholipid. Among sPLA2s from various organisms and tissues, group XIV fungal/bacterial sPLA2s are relatively less characterized compared to their mammalian counterparts. Here we report cloning, recombinant… (More)
DOI: 10.1016/j.pep.2015.08.007

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