Expression, purification, and therapeutic implications of recombinant sFRP1.

@article{Ghoshal2015ExpressionPA,
  title={Expression, purification, and therapeutic implications of recombinant sFRP1.},
  author={Archita Ghoshal and Siddhartha Sankar Ghosh},
  journal={Applied biochemistry and biotechnology},
  year={2015},
  volume={175 4},
  pages={
          2087-103
        }
}
Secreted frizzled-related proteins (sFRPs) constitute a family of proteins, which impede the Wnt signaling pathway. Upregulation of the Wnt cascade is one of the multiple facets of carcinogenesis. Herein, we report the expression, solubilization, purification, characterization, and anti-cell proliferative activity of a novel recombinant GST-tagged sFRP1 of human origin. sFRP1 was cloned into pGEX-4T2 bacterial expression vector, and the recombinant protein was overexpressed in Escherichia coli… CONTINUE READING
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