Expression, purification, and refolding of recombinant fusion protein hIL-2/mGM-CSF.

@article{Wen2008ExpressionPA,
  title={Expression, purification, and refolding of recombinant fusion protein hIL-2/mGM-CSF.},
  author={Qian Wen and Li Ma and Wei Luo and Ming-qian Zhou and X Wang},
  journal={Biomedical and environmental sciences : BES},
  year={2008},
  volume={21 6},
  pages={
          509-13
        }
}
OBJECTIVE To study the activities of interleukin (IL)-2 and granulocyte-macrophage colony-stimulating factor (GM-CSF) (hIL-2/mGM-CSF). METHODS SOE PCR was used to change the linker of the fusion protein for higher activities. The fusion protein was expressed in Escherichia coli (E. coli) BL21 (DE3) in inclusion body (IB) form. After IB was extracted and clarified, it was denatured and purified by affinity chromatography. The protein was refolded by dilution in a L-arginine refolding buffer… CONTINUE READING