Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum.

@article{Reddy1996ExpressionPA,
  title={Expression, purification, and crystallization of meso-diaminopimelate dehydrogenase from Corynebacterium glutamicum.},
  author={Srinivasa G Reddy and Giovanna Scapin and John S Blanchard},
  journal={Proteins},
  year={1996},
  volume={25 4},
  pages={514-6}
}
The gene encoding the meso-diaminopimelate dehydrogenase (DAPDH) from Corynebacterium glutamicum was over-expressed and purified to homogeneity. Crystals of the binary DAPDH-NADP+ complex were obtained from solutions of polyethylene glycol 8000, 100 mM sodium cacodylate, pH 6.5, and 150-300 mM Mg(OAc)2. The crystals diffract to 2.2 A, belong to the… CONTINUE READING