Expression, purification, and characterization of human factor XIII in Saccharomyces cerevisiae.

@article{Bishop1990ExpressionPA,
  title={Expression, purification, and characterization of human factor XIII in Saccharomyces cerevisiae.},
  author={Paul D. Bishop and David C. Teller and Richard A. Smith and Gerald Lasser and Teresa L. Gilbert and R. L. Seale},
  journal={Biochemistry},
  year={1990},
  volume={29 7},
  pages={1861-9}
}
Factor XIII is the terminal enzyme of the clotting cascade. A cDNA sequence encoding human placental factor XIII was expressed in Saccharomyces cerevisiae with the yeast ADH2-4c promoter. Expression levels were a strong function of the noncoding flanking DNA content of the construction. When the terminal 3'-flanking noncoding DNA was removed, expression increased approximately 50-fold. The protein was produced in quantity by high-yield fermentation and purified to homogeneity. The recombinant… CONTINUE READING