Expression, mitogenic activity and regulation by growth hormone of growth hormone/insulin-like growth factor in Branchiostoma belcheri

  title={Expression, mitogenic activity and regulation by growth hormone of growth hormone/insulin-like growth factor in Branchiostoma belcheri},
  author={Bin Guo and Shicui Zhang and Shaohui Wang and Yujun Liang},
  journal={Cell and Tissue Research},
The growth hormone (GH)/insulin-like growth factor (IGF) axis is unique to all the vertebrate species but its evolutionary origin is ill-defined. We therefore cloned a cDNA encoding Branchiostoma belcheri IGF (BbIGF). BbIGF was expressed in a tissue-specific manner, with the most abundant expression in the hepatic caecum, the putative liver precursor. The recombinant BbIGF expressed in vitro showed mitogenic activity capable of stimulating cell proliferation in the flounder gill, a… 
The Origination of Growth Hormone/Insulin-Like Growth Factor System: A Story From Ancient Basal Chordate Amphioxus
  • Mengyang Li
  • Biology, Medicine
    Frontiers in Endocrinology
  • 2022
Results reveal the origin of the gene families in the GH/IGF system, providing strong evidence that this system emerged in the amphioxus.
Identification, Evolution and Expression of an Insulin-Like Peptide in the Cephalochordate Branchiostoma lanceolatum
It is hypothesized that ILP has critical implications in both developmental processes and metabolism and could display IGF- and insulin-like functions in amphioxus supporting the idea of a common ancestral protein.
Structural and functional analysis of the amphioxus IGFBP gene uncovers ancient origin of IGF-independent functions.
The results suggest that the nuclear localization and transcription activation activity of IGFBPs are ancient functions and the IGF-binding function may have been acquired by opportunistic gain-of-functional mutations later in evolution.
Identification and expression of liver-specific genes after LPS challenge in amphioxus: the hepatic cecum as liver-like organ and “pre-hepatic” acute phase response
Combining global genome survey and qRT-PCR data sets supports the idea that hepatic cecum in amphioxus is the “pre-hepatic” organ homologous to vertebrate liver and acts as an immunological organ, playing an important role in APR.
Amphioxus muscle transcriptomes reveal vertebrate-like myoblast fusion genes and a highly conserved role of insulin signalling in the metabolism of muscle
This study elucidates the evolutionary trajectory of muscle genes as they relate to the increased complexity of vertebrate muscles and muscle development with significant refining of the ancestral state for chordate myoblast fusion genes.
[Hepatic caecum of amphioxus and origin of vertebrate liver].
Recent advances on the relationship between amphioxus hepatic caecum and the vertebrate liver as well as the origin of growth hormone/insulin-like growth factor (GH/IGF) and thyroid hormone/thyroid hormone receptor (TH/THR) signal pathways are summarized.


Cloning and expression of insulin-like growth factors I and II in the shi drum (Umbrina cirrosa).
Evolution of insulin-like growth factor (IGF) function: production and characterization of recombinant hagfish IGF.
Rec recombinant hagfish IGF is made, indicating that motifs important for functions associated with mammalian IGFs appear to have evolved prior to the Agnathans diverging from the main line of vertebrate evolution 550 million years ago.
Insulin-like growth factors I and II. Peptide, messenger ribonucleic acid and gene structures, serum, and tissue concentrations.
Current progress in the immunological measurement of the IGF is reviewed including different strategies for avoiding binding protein interference and an attempt is made to indicate circumstances in which the IGFs are exerting their actions in an autocrine/paracrine mode and when endocrine actions predominate.
Comparative endocrinology of the insulin-like growth factor-binding protein.
This review highlights selected 'comparative aspects' in current IGFBP research, in an attempt to view this essential group of endocrine regulators from a wider, biological perspective.
Ancient divergence of insulin and insulin-like growth factor.
It is concluded that insulin and IGF have maintained separate gene lineages in both vertebrate and protochordate evolution and, thus, a distinct evolutionary history of more than 600 million years.
Structure and expression of a chicken insulin-like growth factor I precursor.
There is strong amino acid conservation between chicken and mammalian IGF-I throughout the entire protein, suggesting that it functions as the authentic translation initiation site, an observation supported by cell-free studies of biosynthesis and cotranslational proteolytic processing.
Structure and expression of the insulin-like peptide receptor from amphioxus.
It is proposed that the ancestral ILP receptor gene also duplicated and diverged to generate the IR and IGF-IR genes during the evolutionary transition from protochordates to vertebrates.
The phylogeny of the insulin-like growth factors.
IGFs stimulate zebrafish cell proliferation by activating MAP kinase and PI3-kinase-signaling pathways.
It is shown that both IGF-I and -II are potent mitogens for zebrafish embryonic cells and that activation of both the MAPK and PI3 kinase-signaling pathways is required for the mitogenic action of IGFs in zebra fish embryonic cells.