Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Xanthomonas oryzae pv. oryzae.

@article{Ngo2008ExpressionCA,
  title={Expression, crystallization and preliminary X-ray crystallographic analysis of peptide deformylase from Xanthomonas oryzae pv. oryzae.},
  author={Phuong-Thuy Ho Ngo and Jin-Kwang Kim and Hyesoon Kim and Junho Jung and Yeh-Jin Ahn and Jeong-Gu Kim and Byoung-Moo Lee and Lin-Woo Kang},
  journal={Acta crystallographica. Section F, Structural biology and crystallization communications},
  year={2008},
  volume={64 Pt 11},
  pages={1031-3}
}
Peptide deformylase (PDF) catalyzes the removal of the N-formyl group from the N-terminus of newly synthesized polypeptides; this process is crucial for cell survival. As it is an antibacterial drug target against Xanthomonas oryzae pv. oryzae (Xoo), PDF from Xoo was cloned, expressed, purified and crystallized. Native PDF crystals diffracted to 2.7 A resolution and belonged to the hexagonal space group P6(1)22, with unit-cell parameters a = b = 59.0, c = 266.3 A. One monomer is present in the… CONTINUE READING