Expression, crystallization, and three-dimensional structure of the catalytic domain of human plasma kallikrein.

@article{Tang2005ExpressionCA,
  title={Expression, crystallization, and three-dimensional structure of the catalytic domain of human plasma kallikrein.},
  author={Jie Tang and Christine Luong Yu and Steven R. Williams and Eric B Springman and D. Ross Jeffery and Paul A. Sprengeler and Alberto Fragoso Est{\'e}vez and Jun Sampang and William D Shrader and Jeff Spencer and Wendy Young and Mary Kay McGrath and Bradley A. Katz},
  journal={The Journal of biological chemistry},
  year={2005},
  volume={280 49},
  pages={41077-89}
}
Plasma kallikrein is a serine protease that has many important functions, including modulation of blood pressure, complement activation, and mediation and maintenance of inflammatory responses. Although plasma kallikrein has been purified for 40 years, its structure has not been elucidated. In this report, we described two systems (Pichia pastoris and baculovirus/Sf9 cells) for expression of the protease domain of plasma kallikrein, along with the purification and high resolution crystal… CONTINUE READING