Expression, characterization, and crystallization of a member of the novel phospholipase D family of phosphodiesterases.

@article{Zhao1997ExpressionCA,
  title={Expression, characterization, and crystallization of a member of the novel phospholipase D family of phosphodiesterases.},
  author={Yang Zhao and Jeanne A. Stuckey and Daniel L. Lohse and Jack E. Dixon},
  journal={Protein science : a publication of the Protein Society},
  year={1997},
  volume={6 12},
  pages={2655-8}
}
A family of phospholipase D (PLD) proteins has recently been identified (Koonin, 1996; Ponting & Kerr, 1996) based upon amino acid sequence identity. This family includes human and plant PLDs, proteins encoded by open reading frames in pathogenic viruses and bacteria, as well as an endonuclease. The endonuclease, known as Nuc, is encoded by the IncN plasmid, pKM101, present in Salmonella typhimurium. The recombinant Nuc protein has been expressed and purified from Escherichia coli. The amino… CONTINUE READING