Expression, characterization, and crystallization of the pyrophosphate-dependent phosphofructo-1-kinase of Borrelia burgdorferi.

@article{Deng1999ExpressionCA,
  title={Expression, characterization, and crystallization of the pyrophosphate-dependent phosphofructo-1-kinase of Borrelia burgdorferi.},
  author={Z Deng and david. roberts and X Wang and Robert G. Kemp},
  journal={Archives of biochemistry and biophysics},
  year={1999},
  volume={371 2},
  pages={326-31}
}
The two genes for the putative pyrophosphate-dependent phosphofructokinases (PPi-PFKs) of Borrelia burgdorferi were cloned by PCR and expressed in Escherichia coli, and their protein products were purified to near homogeneity. The larger of the two gene products, a 62-kDa protein, is an active PPi-PFK and exists in solution as a dimer. It has apparent K(m… CONTINUE READING