Expression, Purification and Characterization of Recombinant Canine FGF21 in Escherichia coli.

@article{Zheng2016ExpressionPA,
  title={Expression, Purification and Characterization of Recombinant Canine FGF21 in Escherichia coli.},
  author={Zhong Zheng and Chengjun Yang and Ruofeng Yin and Jinxi Jiang and Haiting He and Xinxin Wang and Mujie Kan and Yechen Xiao},
  journal={Protein and peptide letters},
  year={2016},
  volume={23 9},
  pages={
          785-9
        }
}
The canine metabolic diseases, such as obesity and diabetes, have become a worldwide problem. Fibroblast growth factor 21 (FGF21) is a potent regulator which has many biological functions relative to metabolism regulation. It suggests that FGF21 plays important roles in regulating canine metabolic diseases. To acquire the recombinant canine FGF21 (rcFGF21) in Escherichia coli, the recombinant bacteria were induced by 0.5 mM IPTG for 16 hours at 16 °C, and the rcFGF21 protein was purified by Ni… CONTINUE READING
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