Expressing and bioactivity analysis of shark hepatic stimulate substance analogue in Escherichia coli

@inproceedings{Min2004ExpressingAB,
  title={Expressing and bioactivity analysis of shark hepatic stimulate substance analogue in Escherichia coli},
  author={Wang Rong Min},
  year={2004}
}
After being induced with1mmol/LIPTG,the recombinational protein was purified by Gel chromotograˉbing and the activity analyzed with MTT,the prokargotic expression plasmid of sharks hepatic stimulant analogues gene was constructed and transformed into Escherichia coli BLZL line. The results showthat the quantities of recombinant protein to the about38%of the total soluble proteins of BL21under the induction of IPTG,its molecular weight is about17500u analyzed by SDS-PAGE electrophoresis.The… CONTINUE READING

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