Expressed-sequence-tag approach to identify differentially expressed genes following peripheral nerve axotomy

@article{Tanabe1998ExpressedsequencetagAT,
  title={Expressed-sequence-tag approach to identify differentially expressed genes following peripheral nerve axotomy},
  author={Katsuhisa Tanabe and Saya Nakagomi and Sumiko Kiryu-Seo and Yuji Imai and Hiroshi Kiyama},
  journal={Neuroscience Research},
  year={1998},
  volume={31}
}
Gene expression profiles in the rat hypoglossal nucleus after axotomy were demonstrated using expressed-sequence-tag (EST) approach. To demonstrate the gene-expression profiles after axotomy, nerve-transected hypoglossal nuclei were dissected and collected from about 1000 rats, with which a cDNA library was constructed. More than 750 clones were sub-cloned and sequenced from the library. The clones which hit frequently are likely to be associated with mitochondrial respiratory chain… CONTINUE READING

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