Expressed Repeat Elements Improve RT-qPCR Normalization across a Wide Range of Zebrafish Gene Expression Studies

@inproceedings{Vanhauwaert2014ExpressedRE,
  title={Expressed Repeat Elements Improve RT-qPCR Normalization across a Wide Range of Zebrafish Gene Expression Studies},
  author={Suzanne Vanhauwaert and Gert Van Peer and Ali Rihani and Els D R Janssens and Pieter Rondou and Steve Lefever and Anne De Paepe and Paul J Coucke and Frank Speleman and Jo Vandesompele and Andy Willaert and Nicholas Simon Foulkes},
  booktitle={PloS one},
  year={2014}
}
The selection and validation of stably expressed reference genes is a critical issue for proper RT-qPCR data normalization. In zebrafish expression studies, many commonly used reference genes are not generally applicable given their variability in expression levels under a variety of experimental conditions. Inappropriate use of these reference genes may lead to false interpretation of expression data and unreliable conclusions. In this study, we evaluated a novel normalization method in… CONTINUE READING