Expressed Alu repeats as a novel, reliable tool for normalization of real-time quantitative RT-PCR data

Abstract

We describe a novel strategy for mRNA normalization in quantitative real-time PCR that is based on expressed Alu repeat amplification as a measure for the mRNA fraction. We show that expressed Alu repeat amplification is a fast, accurate normalization tool that can be successfully used for quantification of selected mRNA in the human transcriptome. This… (More)
DOI: 10.1186/gb-2010-11-1-r9

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