Exposure to artemisinin at the trophozoite stage increases sexual conversion rates in the malaria parasite Plasmodium falciparum

  title={Exposure to artemisinin at the trophozoite stage increases sexual conversion rates in the malaria parasite Plasmodium falciparum},
  author={H. P. Portugaliza and S. Miyazaki and Fiona J. A. Geurten and C. Pell and A. Rosanas-Urgell and C. Janse and Alfred Cort{\'e}s},
Malaria transmission is dependent on formation of gametocytes in the human blood. The sexual conversion rate, the proportion of asexual parasites that convert into gametocytes at each multiplication cycle, is variable and reflects the relative parasite investment between transmission and maintaining the infection. The impact of environmental factors such as drugs on sexual conversion rates is not well understood. We developed a robust assay using gametocyte-reporter parasite lines to accurately… Expand
2 Citations
Single-Cell RNA Sequencing Reveals Cellular Heterogeneity and Stage Transition under Temperature Stress in Synchronized Plasmodium falciparum Cells
In this study, it is found that parasites exhibit transcriptional heterogeneity in an otherwise morphologically synchronized culture, and a subset of parasites is continually committed to gametocytogenesis and stress-responsive pathways. Expand
Single-Cell RNA Sequencing Reveals Cellular Heterogeneity and Stage Transition under Temperature Stress in Synchronized <named-content content-type='genus-species'>Plasmodium falciparum</named-content> Cells
The malaria parasite has a complex life cycle exhibiting phenotypic and morphogenic variations in two different hosts by existing in heterogeneous developmental states. To investigate this cellularExpand


Plasmodium chabaudi: effect of antimalarial drugs on gametocytogenesis.
The data suggest that antimalarial drugs may frequently have only a small effect on reducing malaria transmission and may help to explain the rapid spread of drug-resistant geno-types. Expand
Chloroquine increases Plasmodium falciparum gametocytogenesis in vitro.
A 5-fold increase in gametocytogenesis is demonstrated in the human malaria, P. falciparum, in vitro, in response to treatment with the antimalarial drug chloroquine, and there were clone differences in the relationship between stress and gametocyte production, implying the response was genetically variable. Expand
An assay to probe Plasmodium falciparum growth, transmission stage formation and early gametocyte development
An assay designed to probe the effect of drugs or other perturbations on asexual replication, sexual conversion and early gametocyte development in the major human malaria parasite Plasmodium falciparum is described. Expand
Reporter lines based on the gexp02 promoter enable early quantification of sexual conversion rates in the malaria parasite Plasmodium falciparum
A sexual conversion assay that shortens considerably the time needed for an accurate determination of sexual conversion rates, and dispenses the need to add chemicals to inhibit parasite replication is developed. Expand
Lysophosphatidylcholine Regulates Sexual Stage Differentiation in the Human Malaria Parasite Plasmodium falciparum
It is shown that the host-derived lipid lysophosphatidylcholine (LysoPC) controls P. falciparum cell fate by repressing parasite sexual differentiation, and a major component of the sexual differentiation pathway in Plasmodium is introduced that may provide new approaches for blocking malaria transmission. Expand
Upregulation of gametocytogenesis in anti-malarial drug-resistant Plasmodium falciparum
  • T. Rajapandi
  • Biology, Medicine
  • Journal of Parasitic Diseases
  • 2019
It was found that exposure of an asynchronous culture of artemisinin-resistant P. falciparum IPC 5202 to DHA, a line carrying a mutation in the PfK13 gene that is linked to artemis inin resistance, results in dormancy, and this is in contrast to the resistant parasites exposed to a second dose of art Artemisinin, which resulted in a very high rate of dormancy and recovery into sexual forms or gametocytes. Expand
Adaptive changes in Plasmodium transmission strategies following chloroquine chemotherapy
If surviving parasites produce compensatory increases in their rate of gametocyte production similar to those reported here, such treatment may have minimal effect on decreasing, and may actually increase, transmission. Expand
Revisiting the initial steps of sexual development in the malaria parasite Plasmodium falciparum
Human to vector transmission of malaria requires that some blood-stage parasites abandon asexual growth and convert into non-replicating sexual forms called gametocytes. The initial steps ofExpand
Effects of artemisinin derivatives on malaria transmissibility
The introduction of the artemisinin derivatives in routine treatment at this study site in mid 1994 was associated with a reduction in the subsequent incidence of falciparum malaria and may prevent the spread of multidrug resistance. Expand
Routine in vitro culture of P. falciparum gametocytes to evaluate novel transmission-blocking interventions
It is shown how functionally viable gametocytes can be used to evaluate transmission-blocking drugs both in a field setting and at high throughput (HTP) for drug discovery. Expand