Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome.

@article{KatzJaffe2008ExposureOM,
  title={Exposure of mouse oocytes to 1,2-propanediol during slow freezing alters the proteome.},
  author={Mandy G. Katz-Jaffe and Mark G. Larman and Courtney B Sheehan and David K. Gardner},
  journal={Fertility and sterility},
  year={2008},
  volume={89 5 Suppl},
  pages={1441-7}
}
OBJECTIVE To examine the effect of exposure to 1,2-propanediol (PrOH) on the mammalian oocyte proteome. DESIGN Experimental study. SETTING Research laboratory. ANIMAL(S) Four- to five-week-old F1 (C57BL/6 x CBA/Ca) female mice. INTERVENTION(S) Analysis by time-of-flight mass spectrometry. MAIN OUTCOME MEASURE(S) Protein profiles of denuded mouse metaphase II (MII) oocytes. RESULT(S) Metaphase II oocytes exposed to 1.5 M PrOH for 20 minutes with or without seeding (i.e… CONTINUE READING