Exposure of human lung cells to inhalable substances: a novel test strategy involving clean air exposure periods using whole diluted cigarette mainstream smoke.

@article{Ritter2003ExposureOH,
  title={Exposure of human lung cells to inhalable substances: a novel test strategy involving clean air exposure periods using whole diluted cigarette mainstream smoke.},
  author={Detlef Ritter and Jan W Knebel and Michaela Aufderheide},
  journal={Inhalation toxicology},
  year={2003},
  volume={15 1},
  pages={67-84}
}
An experimental approach was established for the validation of an in vitro test system for complex environmental test atmospheres consisting of both gaseous substances and particulates. Smoke from two different cigarette types (generated by an automatic cigarette-smoking machine) was employed to assess both the sensitivity and the specificity of the system. The smoke was diluted with synthetic air and used to expose human lung cells grown on microporous membranes. Cells were exposed alternately… CONTINUE READING
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