Exposure of cryptic epitopes on transthyretin only in amyloid and in amyloidogenic mutants.

@article{Goldsteins1999ExposureOC,
  title={Exposure of cryptic epitopes on transthyretin only in amyloid and in amyloidogenic mutants.},
  author={Gundars Goldsteins and H{\aa}kan Persson and Kjell Andersson and Anders Olofsson and Ingrid Dacklin and A A Edvinsson and Maria Jo{\~a}o Saraiva and Erik Lundgren},
  journal={Proceedings of the National Academy of Sciences of the United States of America},
  year={1999},
  volume={96 6},
  pages={3108-13}
}
The structural requirements for generation of amyloid from the plasma protein transthyretin (TTR) are not known, although it is assumed that TTR is partly misfolded in amyloid. In a search for structural determinants important for amyloid formation, we generated a TTR mutant with high potential to form amyloid. We demonstrated that the mutant represents an intermediate in a series of conformational changes leading to amyloid. Two monoclonal antibodies were generated against this mutant; each… CONTINUE READING