Export of cytochrome P450 105D1 to the periplasmic space of Escherichia coli.

@article{Kaderbhai2001ExportOC,
  title={Export of cytochrome P450 105D1 to the periplasmic space of Escherichia coli.},
  author={Mustak A. Kaderbhai and Cynthia C. Ugochukwu and Steven L Kelly and David Christopher Lamb},
  journal={Applied and environmental microbiology},
  year={2001},
  volume={67 5},
  pages={2136-8}
}
CYP105D1, a cytochrome P450 from Streptomyces griseus, was appended at its amino terminus to the secretory signal of Escherichia coli alkaline phosphatase and placed under the transcriptional control of the native phoA promoter. Heterologous expression in E. coli phosphate-limited medium resulted in abundant synthesis of recombinant CYP105D1 that was translocated across the bacterial inner membrane and processed to yield authentic, heme-incorporated P450 within the periplasmic space. Cell… CONTINUE READING