Export of a protein into the outer membrane of Escherichia coli K12. Stable incorporation of the OmpA protein requires less than 193 amino-terminal amino-acid residues.

@article{Bremer1982ExportOA,
  title={Export of a protein into the outer membrane of Escherichia coli K12. Stable incorporation of the OmpA protein requires less than 193 amino-terminal amino-acid residues.},
  author={Erhard Bremer and Stewart T Cole and Ingrid Hindennach and U. Henning and Ewald Beck and Ch. Kurz and Hubert Schaller},
  journal={European journal of biochemistry},
  year={1982},
  volume={122 1},
  pages={223-31}
}
The cloned ompA gene encoding the major outer membrane protein OmpA of Escherichia coli has been shortened in vitro by exonuclease digestion from the end corresponding to the CO2H terminus of the protein. Nine derivatives were identified which still possessed substantial parts of the ompA gene and one was constructed which had suffered a small deletion early in the gene. Gene fragments encoding NH2-terminal OmpA sequences of 45, 133, 193, and 227 residues of the 325 amino acids of OmpA were… CONTINUE READING

Citations

Publications citing this paper.
Showing 1-10 of 16 extracted citations

Recombinant outer membrane protein A fragments protect against Escherichia coli meningitis.

Journal of microbiology, immunology, and infection = Wei mian yu gan ran za zhi • 2016
View 1 Excerpt

Similar Papers

Loading similar papers…