Expolygalacturonase from Suspension Cultures of Marchantia polymorpha: Its Presence and Involvement in Pectic Polysaccharide Degradation.

@article{Konno1983ExpolygalacturonaseFS,
  title={Expolygalacturonase from Suspension Cultures of Marchantia polymorpha: Its Presence and Involvement in Pectic Polysaccharide Degradation.},
  author={Hiroshi Konno and Yoshiki Yamasaki and Kenji Katoh},
  journal={Plant physiology},
  year={1983},
  volume={73 2},
  pages={216-22}
}
Polygalacturonase was isolated from cell suspension cultures of a thalloid liverwort, Marchantia polymorpha. The enzyme in the ;buffer-soluble' protein fraction was dialyzed at pH 5.2 and further purified 91-fold by a combination of chromatographic techniques including CM-Sephadex, Sephacryl S-200, DEAE-Sephadex, and Sephadex G-200. The purified enzyme had an optimum activity in the pH range at 3.6 to 3.8 and molecular weight of 76,000 daltons, and its activity was not stimulated by cations… CONTINUE READING