Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance

@article{FrauenkronMachedjou2018ExploringTF,
  title={Exploring the full natural diversity of single amino acid exchange reveals that 40–60% of BSLA positions improve organic solvents resistance},
  author={Victorine Josiane Frauenkron-Machedjou and A. Fulton and J. Zhao and L. Weber and K. Jaeger and U. Schwaneberg and Leilei Zhu},
  journal={Bioresources and Bioprocessing},
  year={2018},
  volume={5},
  pages={1-12}
}
  • Victorine Josiane Frauenkron-Machedjou, A. Fulton, +4 authors Leilei Zhu
  • Published 2018
  • Chemistry
  • Bioresources and Bioprocessing
  • ObjectivesProtein engineering has been employed to successfully improve organic solvent resistance of enzymes. Exploration of nature’s full potential (how many beneficial positions/beneficial substitutions of the target enzyme) to improve organic solvent resistance of enzymes by a systematic study was performed.ResultsWe report the results of screening the previously generated BSLA (Bacillus subtilis lipase A)-SSM (site saturation mutagenesis) library (covering the full natural diversity of… CONTINUE READING
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