Exploring the Development of Ground-State Destabilization and Transition-State Stabilization in Two Directed Evolution Paths of Kemp Eliminases.

Abstract

Computer-aided enzyme design presents a major challenge since in most cases it has not resulted in an impressive catalytic power. The reasons for the problems with computational design include the use of nonquantitative approaches, but they may also reflect other difficulties that are not completely obvious. Thus, it is very useful to try to learn from the… (More)
DOI: 10.1021/acscatal.7b00171

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Cite this paper

@article{Jindal2017ExploringTD, title={Exploring the Development of Ground-State Destabilization and Transition-State Stabilization in Two Directed Evolution Paths of Kemp Eliminases.}, author={Garima Jindal and Balajee Ramachandran and Ram Prasad Bora and Arieh Warshel}, journal={ACS catalysis}, year={2017}, volume={7 5}, pages={3301-3305} }