Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis.

@article{Akinc2005ExploringPD,
  title={Exploring polyethylenimine-mediated DNA transfection and the proton sponge hypothesis.},
  author={Akin Akinc and Mini P. Thomas and Alexander M. Klibanov and Robert Langer},
  journal={The journal of gene medicine},
  year={2005},
  volume={7 5},
  pages={
          657-63
        }
}
BACKGROUND The relatively high transfection efficiency of polyethylenimine (PEI) vectors has been hypothesized to be due to their ability to avoid trafficking to degradative lysosomes. According to the proton sponge hypothesis, the buffering capacity of PEI leads to osmotic swelling and rupture of endosomes, resulting in the release of the vector into the cytoplasm. METHODS The mechanism of PEI-mediated DNA transfer was investigated using quantitative methods to study individual steps in the… CONTINUE READING
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