Exploring directional invasion of serum nuclease into siRNA duplexes by asymmetrical terminal modifications.

Abstract

In this study we demonstrated that ribonuclease A (RNase A) can recognize the thermodynamic asymmetry of siRNA duplexes, similar to other proteins involved in siRNA function such as argonaute 2. RNase A preferentially invades the siRNA duplex through the less stable terminus, i.e., the 3' terminus of the sense strand. As evidence, only phosphorothioate (PS… (More)
DOI: 10.1002/cmdc.201402115

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@article{Huang2014ExploringDI, title={Exploring directional invasion of serum nuclease into siRNA duplexes by asymmetrical terminal modifications.}, author={Ye Huang and Yuan Ma and Yujia Guo and Lang Zou and Hongwei Jin and Lijun Zhong and Yun Wu and Lihe Zhang and Zhenjun Yang}, journal={ChemMedChem}, year={2014}, volume={9 9}, pages={2111-9} }