Exploring diet-dependent shifts in methanogen and methanotroph diversity in the rumen of Mehsani buffalo by a metagenomics approach

  title={Exploring diet-dependent shifts in methanogen and methanotroph diversity in the rumen of Mehsani buffalo by a metagenomics approach},
  author={Nidhi Parmar and J. I. Nirmal Kumar and Chaitanya G. Joshi},
  journal={Frontiers in Life Science},
  pages={371 - 378}
In this study, metagenomics was applied to characterize microbial communities, specifically methanogens and methanotrophs, and to discover their functional activities under two different dietary treatments. To retrieve an overall rumen microbial community profile and to check the abundance of methanogenic and methanotrophic bacteria therein, semiconductor shotgun sequencing of DNA isolated from the rumen fluid of Mehsani buffalo treated with two different diets, i.e. 50% green roughage/50… 
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