Exploring Microbial Diversity and Taxonomy Using SSU rRNA Hypervariable Tag Sequencing


Massively parallel pyrosequencing of hypervariable regions from small subunit ribosomal RNA (SSU rRNA) genes can sample a microbial community two or three orders of magnitude more deeply per dollar and per hour than capillary sequencing of full-length SSU rRNA. As with full-length rRNA surveys, each sequence read is a tag surrogate for a single microbe… (More)
DOI: 10.1371/journal.pgen.1000255


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