Exploratory Study on Th1 Epitope-Induced Protective Immunity against Coxiella burnetii Infection

@article{Xiong2014ExploratorySO,
  title={Exploratory Study on Th1 Epitope-Induced Protective Immunity against Coxiella burnetii Infection},
  author={Xiaolu Xiong and Yong Qi and Jun Jiao and Wenping Gong and Changsong Duan and Bohai Wen},
  journal={PLoS ONE},
  year={2014},
  volume={9}
}
Coxiella burnetii is a Gram-negative bacterium that causes Q fever in humans. In the present study, 131 candidate peptides were selected from the major immunodominant proteins (MIPs) of C. burnetii due to their high-affinity binding capacity for the MHC class II molecule H2 I-Ab based on bioinformatic analyses. Twenty-two of the candidate peptides with distinct MIP epitopes were well recognized by the IFN-γ recall responses of CD4+ T cells from mice immunized with parental proteins in an… 

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References

SHOWING 1-10 OF 34 REFERENCES
Identification of CD4+ T Cell Epitopes in C. burnetii Antigens Targeted by Antibody Responses
TLDR
A surprisingly non-uniform pattern is found in the help provided by epitope specific CD4+ T cells for antibody production, which suggests that a complete map ofCD4+ response targets in PI-WCV vaccinated mice will likely include antigens against which no antibody responses are made.
Identification and Cloning of Immunodominant Antigens of Coxiella burnetii
TLDR
The results suggest that several specific immunogenic antigens may play critical roles in the development of protective immunity and that they are logical candidates for vaccine and serodiagnostic reagents.
Development of a Lipopolysaccharide-Targeted Peptide Mimic Vaccine against Q Fever
TLDR
The results indicate that m1E41920-KLH was able to confer significant protection against C. burnetii challenge and is a protective Ag and may be useful for developing a safe and effective vaccine against Q fever.
Mechanisms of Vaccine-Induced Protective Immunity against Coxiella burnetii Infection in BALB/c Mice1
TLDR
Novel evidence is presented to highlight the importance of PI-LPS and Abs in protective immunity and has important implications for the design of new generation vaccines against Q fever.
Polyfunctional CD4+ T Cell Responses to Immunodominant Epitopes Correlate with Disease Activity of Virulent Salmonella
TLDR
The identification of novel CD4+ T cell peptide epitopes, which are conserved among Salmonella serovars, are reported, which could contribute to improved diagnosis ofSalmonella-related diseases and rational design of Salmoneella vaccines.
Components of protective immunity.
TLDR
New information related to the characterization of acquired immunity to C. burnetii vaccination and infection is described that will provide a fundamental understanding of the development of protective immunity against Q fever.
Coxiella burnetii antigen-stimulated dendritic cells mediated protection against Coxiella burnetii in BALB/c mice.
TLDR
It is suggested that PIIAg is far superior to PIAg in activating BMDCs to confer protection against C. burnetii in vivo, whereas Com1 and SecB are protective antigens because Com1- or SecB-pulsed BM DCs confer partial protection.
Vaccines against Coxiella infection
TLDR
New information relating to the characterization of acquired immunity to C. burnetii infection is described that will provide a fundamental understanding of the development of protective immunity against Q fever.
Genome‐wide profiling of humoral immune response to Coxiella burnetii infection by protein microarray
TLDR
Nine of the differentially reactive antigens identified were validated on an alternative immunostrip platform, demonstrating proof‐of‐concept development of a consistent, safe, and inexpensive diagnostic assay alternative.
Antigenic analysis for vaccines and diagnostics.
TLDR
The search for new diagnostic reagents and vaccines has utilized new methods for discovery of immunoreactive proteins in Coxiella burnetii, and microarrays have allowed the analysis of peptide libraries of open reading frames to evaluate the immunogenicity of complete genomes.
...
1
2
3
4
...