Exploration of the Functional Domains of H - NS in Escherichia coli via Mutagenesis

Abstract

.................................................................................................................... 3 Introduction ............................................................................................................... 5 Chapter 1: H-NS Mutations ...................................................................................... 8 Introduction ................................................................................................................................. 8 Methods ....................................................................................................................................... 9 Site-Directed Mutagenesis ....................................................................................................... 9 Chromosomal mutants ............................................................................................................. 9 Results ....................................................................................................................................... 13 Discussion .................................................................................................................................. 14 Chapter 2. H-NS Overexpression ........................................................................... 16 Introduction ............................................................................................................................... 16 Methods ..................................................................................................................................... 17 Plating assay ........................................................................................................................... 17 Visualization of Mutants ........................................................................................................ 17 Results ....................................................................................................................................... 18 Discussion .................................................................................................................................. 23 Chapter 3: Repression ............................................................................................. 26 Introduction ............................................................................................................................... 26 Methods ..................................................................................................................................... 27 hdeA repression Assay ........................................................................................................... 27 Swarming Assay .................................................................................................................... 28 Results ....................................................................................................................................... 29 Discussion .................................................................................................................................. 31 Conclusions ............................................................................................................. 33 Appendix I. DNA Damage ..................................................................................... 35 Introduction ............................................................................................................................... 35 Methods ..................................................................................................................................... 36 Plating assay ........................................................................................................................... 36 Results and Discussion .............................................................................................................. 37 Acknowledgments ................................................................................................... 38

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Cite this paper

@inproceedings{Franco2015ExplorationOT, title={Exploration of the Functional Domains of H - NS in Escherichia coli via Mutagenesis}, author={Charlotte Franco and Susan T Lovett}, year={2015} }