Exploration of Fluorescent Protein Voltage Probes Based on Circularly Permuted Fluorescent Proteins

  title={Exploration of Fluorescent Protein Voltage Probes Based on Circularly Permuted Fluorescent Proteins},
  author={Sunita Ghimire Gautam and Am{\'e}lie Perron and Hiroki Mutoh and Thomas Kn{\"o}pfel},
  booktitle={Front. Neuroeng.},
Genetically encoded fluorescent protein (FP) voltage sensors are promising tools for optical monitoring of the electrical activity of cells. Over the last decade, several designs of fusion proteins have been explored and some of them have proven to be sensitive enough to record membrane voltage transients from single mammalian cells. Most prominent are the families of voltage sensitive fluorescent proteins (VSFPs) that utilize the voltage sensor domain (VSD) of Ciona intestinalis voltage sensor… CONTINUE READING

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