Exploiting viral cell-targeting abilities in a single polypeptide, non-infectious, recombinant vehicle for integrin-mediated DNA delivery and gene expression.

@article{Ars2000ExploitingVC,
  title={Exploiting viral cell-targeting abilities in a single polypeptide, non-infectious, recombinant vehicle for integrin-mediated DNA delivery and gene expression.},
  author={Anna Ar{\'i}s and Jordi Feliu and Andrew Knight and Charles Coutelle and Antonio Villaverde},
  journal={Biotechnology and bioengineering},
  year={2000},
  volume={68 6},
  pages={689-96}
}
A recombinant, multifunctional protein has been designed for optimized, cell-targeted DNA delivery and gene expression in mammalian cells. This hybrid construct comprises a viral peptide ligand for integrin alpha(V)beta(3) binding, a DNA-condensing poly-L-lysine domain, and a complete, functional beta-galactosidase protein that serves simultaneously as purification tag and DNA-shielding agent. This recombinant protein is stable; it has been produced successfully in Escherichia coli and can be… CONTINUE READING
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