Exploiting the complementary nature of LC/MALDI/MS/MS and LC/ESI/MS/MS for increased proteome coverage.

@article{Bodnar2003ExploitingTC,
  title={Exploiting the complementary nature of LC/MALDI/MS/MS and LC/ESI/MS/MS for increased proteome coverage.},
  author={Wanda Bodnar and Robert Kevin Blackburn and Jo M Krise and M. Arthur Moseley},
  journal={Journal of the American Society for Mass Spectrometry},
  year={2003},
  volume={14 9},
  pages={971-9}
}
The goal of this work was to evaluate the improvement in proteome coverage of complex protein mixtures gained by analyzing samples using both LC/ESI/MS/MS and LC/MALDI/MS/MS. Parallel analyses of a single sample were accomplished by interfacing a Probot fractionation system with a nanoscale LC system. The Probot was configured to perform a post-column split such that a fraction (20%) of the column effluent was sent for on-line LC/ESI/MS/MS data acquisition, and the majority of the sample (80… CONTINUE READING