Exploiting the 21st amino acid—purifying and labeling proteins by selenolate targeting


Selenium is essential to human life and occurs in selenoproteins as selenocysteine (Sec), the 21st amino acid. The selenium atom endows selenocysteine with unique biochemical properties, including a low pKa and a high reactivity with many electrophilic agents. Here we describe the introduction of selenocysteine into recombinant non-selenoproteins produced… (More)
DOI: 10.1038/nmeth707


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