Exploitation of specific properties of trifluoroethanol for extraction and separation of membrane proteins.

@article{Deshusses2003ExploitationOS,
  title={Exploitation of specific properties of trifluoroethanol for extraction and separation of membrane proteins.},
  author={Jacques P M Deshusses and Jennifer A. Burgess and Alexander Scherl and Yvan Wenger and Nadia Walter and V{\'e}ronique Converset and Salvo Paesano and Garry L. Corthals and Denis F. Hochstrasser and Jean-Charles Sanchez},
  journal={Proteomics},
  year={2003},
  volume={3 8},
  pages={1418-24}
}
Hydrophobic proteins are difficult to analyze by two-dimensional electrophoresis (2-DE) because of their intrinsic tendency to self-aggregate during the first dimension (isoelectric focusing, IEF) or the equilibration steps. This aggregation renders their redissolution for the second dimension uncertain and results in the reduction of the number and… CONTINUE READING