Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus

  title={Exploitation of Malus EST-SSRs and the utility in evaluation of genetic diversity in Malus and Pyrus},
  author={Lihua Yao and Xiaoyan Zheng and Danying Cai and Yuan Gao and Kun Wang and Yufen Cao and Yuanwen Teng},
  journal={Genetic Resources and Crop Evolution},
A total of 8117 suitable SSR-contaning ESTs were acquired by screening from a Malus EST database, among which dinudeotide SSRs were the most abundant repeat motif, within which, CT/TC followed by AG/GA were predominant. Based on the suitable sequences, we developed 147 SSR primer pairs, of which 94 pairs gave amplifications within the expected size range while 65 pairs were found to be polymorphic after a preliminary test. Eighteen primer pairs selected randomly were further used to assess… CONTINUE READING
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