Explicit Treatment of Non‐Michaelis‐Menten and Atypical Kinetics in Early Drug Discovery **

  title={Explicit Treatment of Non‐Michaelis‐Menten and Atypical Kinetics in Early Drug Discovery **},
  author={Bharath Srinivasan},
Biological systems are highly regulated. They are also highly resistant to sudden perturbations enabling them to maintain the dynamic equilibrium essential to sustain life. This robustness is conferred by regulatory mechanisms that influence the activity of enzymes/proteins within their cellular context to adapt to changing environmental conditions. However, the initial rules governing the study of enzyme kinetics were mostly tested and implemented for cytosolic enzyme systems that were easy to… 
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  • Computer Science, Biology
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  • 2022
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A direct mapping between the printability of the gelatin microgel-gelatin solution based cross-linkable composite bioink and the TG concentration and cross-linking time and the storage modulus, viscosity, and/or process-induced shear stress is established.
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Cost-effective milking plans have been used to supplement the standard supervised four-weekly testing scheme since the 1960s. Cows are typically milked two or more times on a test day, but not all


Michaelis-Menten from an In Vivo Perspective: Open Versus Closed Systems
The Michaelis-Menten system was revisited in which enzymatic turnover, i.e. synthesis and elimination was incorporated, and it was shown that whereas in closed systems there is a threshold for chronic dosing beyond which the substrate concentration keeps rising, in open systems involving enzyme turnover this is no longer the case.
Non-Michaelis-Menten kinetics in cytochrome P450-catalyzed reactions.
  • W. Atkins
  • Biology, Chemistry
    Annual review of pharmacology and toxicology
  • 2005
Divergent approaches, including kinetic modeling and X-ray crystallography, are providing new information about how multiple ligand binding yields complex CYP kinetics.
The Taxonomy of Covalent Inhibitors.
A categorization by mechanism is presented, which highlights common advantages and disadvantages inherent to each approach and helps to facilitate the development of more effective covalent inhibitors.
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Single‐molecule enzymology à la Michaelis–Menten
Both experimental and theoretical techniques that enable single‐molecule analysis are reviewed, with particular emphasis on the major developments in the field of theoretical stochastic enzyme kinetics, from its inception in the mid‐20th century to its modern‐day status.
Allosteric Partial Inhibition of Monomeric Proteases. Sulfated Coumarins Induce Regulation, not just Inhibition, of Thrombin
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Role of substrate unbinding in Michaelis–Menten enzymatic reactions
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