Explant culture of human polycystic kidney.

  title={Explant culture of human polycystic kidney.},
  author={James A. McAteer and Frank A. Carone and J. J. Grantham and Stephen A. Kempson and Kenneth D. Gardner and Andrew P. Evan},
  journal={Laboratory investigation; a journal of technical methods and pathology},
  volume={59 1},
Autosomal dominant polycystic kidney disease is characterized by the formation of large fluid-filled epithelial cysts. To obtain renal cyst wall epithelium for in vitro study, we employed an explant culture technique using medium-hydrated collagen gel as the culture substrate. Pieces of excised cyst wall were submerged within collagen gel. Cells of the cyst lining migrated to form a polarized epithelium at the surface of the surrounding collagen gel. Regions of the outgrowth were isolated by… CONTINUE READING