Experimental therapeutic assays of Tephrosia vogelii against Leishmania major infection in murine model: in vitro and in vivo

  title={Experimental therapeutic assays of Tephrosia vogelii against Leishmania major infection in murine model: in vitro and in vivo},
  author={Sylvia Naliaka Marango and Christopher Khayeka–Wandabwa and Judith Alice Makwali and Bernard N. Jumba and Joseph Kiprop Choge and Eric Onyango Adino and Christopher O. Anjili},
  journal={BMC Research Notes},
BackgroundConventional targeted leishmanicidal chemotherapy has persistently remained prohibitive for most economically deprived communities due to costs, associated time to accessing health services and duration for successful treatment programme. Alternatives are bound to be incorporated in rational management of leishmaniasis by choice or default due to accessibility and cultural beliefs. Therefore, there is need to rigorously investigate and appraise the activity of medicinal compounds that… Expand
In vitro evaluation of the leishmanicidal potential of selected plant-derived extracts against Leishmania (Leishmania) amazonensis
The current standard-of-care for leishmaniasis treatment is unsatisfactory due to are expensive, potentially toxic and long-term treatment requirements, resulting in patient noncompliance. Expand
Murine Models for Development of Anti-Infective Therapeutics
Specific bacterial, fungal, viral, protozoal, and helminth diseases modeled in mouse and rat models for testing the efficacy of a particular drug molecule under study are elaborates on. Expand
Preliminary investigation of acaricidal activity of leaf extract of Nicotiana tabacum on dog tick Rhipicephalus sanguineus
This study investigated the acaricidal activity of methanol and N-hexane leaf extracts of Nicotiana tabacum against dog ticks – Rhipicephalus sanguineus and observed a tick mortality rate that was concentration-dependent. Expand
Isolation of the Tephrosia vogelii extract and rotenoids and their toxicity in the RTgill-W1 trout cell line and in zebrafish embryos.
Results indicate that the crude T. Vogelii extracts are highly potent and the bioactivity of these extracts warrant further investigation for their potential use to treat parasites in human and veterinary medicine and as a natural alternative to pesticides. Expand
Analysis on safety assessment of Tephrosia vogelii Hook to Apis cerana cerana.
The haemolymph of worker bees have the highest content of rotenone, the least accumulation in workers bee's thorax, and the rate of dissipation was slowly during the whole study showed the T. vogelii create security risks to worker bees under some ecosystems. Expand
An enquiry into antileishmanial activity and quantitative analysis of polyhydroxylated steroidal saponins from Solanum paniculatum L. leaves.
An analytical method is developed for the quantification of the eight saponins present in the 70 % ethanol extract from the leaves using ultra-high-performance liquid chromatography coupled to mass spectrometry (UHPLC-MS), showing that saponin 1-3 should be evaluated in further studies for the treatment of cutaneous leishmaniasis. Expand
Phytochemical Analysis of Tephrosia vogelii across East Africa Reveals Three Chemotypes that Influence Its Use as a Pesticidal Plant
Analysis of methanolic extracts of foliar parts of T. vogelii revealed the occurrence of two distinct chemotypes that were separated by the presence of rotenoids in one, and flavanones and flavones that are not bioactive against insects on the other. Expand
Phytochemical Analysis of across East Africa Reveals Three Chemotypes that Influence Its Use as a Pesticidal Plant
Tephrosia vogelii is a plant species chemically characterized by the presence of entomotoxic rotenoids and used widely across Africa as a botanical pesticide. Phytochemical analysis was conducted toExpand


Antileishmanial action of Tephrosia purpurea linn, extract and its fractions against experimental visceral leishmaniasis
The fraction F062 obtained from N‐butanol extract of T. purpurea showed consistent antileishmanial activity at 50 mg/ kg × 5 days by oral route against Leishmania donovani infection in hamsters, confirming activity was further confirmed in a secondary model. Expand
Experimental chemotherapy with Allium sativum (Liliaceae) methanolic extract in rodents infected with Leishmania major and Leishmania donovani.
Since the mechanism of action for the methanolic extract is apparently immunomodulatory, garlic compounds could be purified and tried as complementary medicine in the management of leishmaniases. Expand
Combination therapy using Pentostam and Praziquantel improves lesion healing and parasite resolution in BALB/c mice co-infected with Leishmania major and Schistosoma mansoni
Chemotherapy using the first line of treatment for L. major and S. mansoni reduced the lesion size and parasite loads but did not affect the growth response, spleen and liver, and the use of Pentostam or PZQ did not result in any appreciable disease management. Expand
Experimental therapeutic studies of Solanum aculeastrum Dunal. on Leishmania major infection in BALB/c mice
The results demonstrate that compounds from S. aculeastrum have potential anti-leishmanial activities and the medicinal use of the plant poses considerable toxicity against dividing vero cells. Expand
Activity of antileishmanial agents against amastigotes in human monocyte-derived macrophages and in mouse peritoneal macrophages.
The data suggested that the susceptibilities of organisms to Sb within PEC and HM were generally comparable and that either amastigotes or viable promastigote-induced infections could be used to initiate the infection. Expand
Combination and monotherapy of Leishmania major infection in BALB/c mice using plant extracts and herbicides.
The results of this study demonstrate that combination therapy using alternative administration of saponin, acriflavine, trifluralin and plumbagin is effective in treating L. major infection in mice. Expand
In-vitro Investigation of Fractionated Extracts of Albizia gummifera Seed Against Leishmania donovani Amastigote Stage
The n-butanol and aqueous fractionates of A. gummifera seeds exhibit considerable in-vitro anti L. donovani activity and may be promising anti-leishmanial herbal remedy candidate that support the traditional use of the plant. Expand
Current Treatment of Leishmaniasis: A Review
The aim of this article is to review the current aspects of the pharmacology of leishmaniasis, giving an overview from current agents clinically used to new compounds under development. Expand
Leishmaniasis--current chemotherapy and recent advances in the search for novel drugs.
The immunomodulator imiquimod has proved to be an adjunct for CL therapy and the search for new drugs continues, with bisphosphonates, for example, risedronate and pamidronate, and plant derivatives being reported to have activity against experimental animal infections. Expand
Antileishmanial activity of Aloe Secundiflora plant extracts against Leishmania Major (2013).
Water and methanolic plant extracts significantly inhibited the growth of Leishmania major parasites as compared to amphotericin B with respect to the parasite infection rates and MIC levels. Expand