Experimental test of an analytical model of aberration in an oil-immersion objective lens used in three-dimensional light microscopy.

@article{Gibson1992ExperimentalTO,
  title={Experimental test of an analytical model of aberration in an oil-immersion objective lens used in three-dimensional light microscopy.},
  author={Sarah F. F. Gibson and Frederick Lanni},
  journal={Journal of the Optical Society of America. A, Optics and image science},
  year={1992},
  volume={9 1},
  pages={154-66}
}
Oil-immersion microscope objective lenses have been designed and optimized for the study of thin, two-dimensional object sections that are mounted immediately below the coverslip in a medium that is index matched to the immersion oil. It has been demonstrated both experimentally and through geometrical- and physical-optics theory that, when the microscope is not used with the correct coverslip or immersion oil, when the detector is not located at the optimal plane in image space, or when the… CONTINUE READING
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