Experimental methods for assaying natural transformation and inferring horizontal gene transfer.

@article{Ray2005ExperimentalMF,
  title={Experimental methods for assaying natural transformation and inferring horizontal gene transfer.},
  author={Jessica L Ray and Kaare Magne Nielsen},
  journal={Methods in enzymology},
  year={2005},
  volume={395},
  pages={491-520}
}
The observation of frequent lateral acquisitions of genes in sequenced bacterial genomes has spurred experimental investigations to elucidate the factors governing ongoing gene transfer processes in bacteria. The uptake of naked DNA by natural transformation is known to occur in a wide range of bacterial species and in some archaea. We describe a series of protocols designed to dissect the natural genetic transformability of individual bacterial strains under conditions that progress from… CONTINUE READING