Experimental evolution and gene knockout studies reveal AcrA-mediated isobutanol tolerance in Ralstonia eutropha.

@article{Bernardi2016ExperimentalEA,
  title={Experimental evolution and gene knockout studies reveal AcrA-mediated isobutanol tolerance in Ralstonia eutropha.},
  author={Amanda C Bernardi and Claudia S. Gai and Jingnan Lu and Anthony J Sinskey and Christopher J. Brigham},
  journal={Journal of bioscience and bioengineering},
  year={2016},
  volume={122 1},
  pages={64-9}
}
Isobutanol (IBT) has attracted much attention from researchers as a next generation drop-in biofuel. Ralstonia eutropha is a gram-negative bacterium which naturally produces polyhydroxybutyrate (PHB), and has been reported to produce IBT after metabolic engineering. Similar to other microbes, R. eutropha experiences toxicity from branched-chain alcohols and is unable to grow in the presence of IBT concentrations higher than 0.5% (v v(-1)). Such low tolerance greatly limits the ability of R… CONTINUE READING